link to berkeleygenomics.org methods for strong germline engineeringHEADmaster

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+[berkeleygenomics.org primer on methods of germline engineering](https://berkeleygenomics.org/articles/Methods_for_strong_human_germline_engineering.html):  statistics of chromosome selection, number of polygenic gene edits/iterations required for a desired IQ increase
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 "Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage" <http://pubmedcentralcanada.ca/pmcc/articles/PMC4873371/>
 
 "Here we report the development of base editing, a new approach to genome editing that enables the direct, irreversible conversion of one target DNA base into another in a programmable manner, without requiring dsDNA backbone cleavage or a donor template. We engineered fusions of CRISPR/Cas9 and a cytidine deaminase enzyme that retain the ability to be programmed with a guide RNA, do not induce dsDNA breaks, and mediate the direct conversion of cytidine to uridine, thereby effecting a C→T (or G→A) substitution. The resulting “base editors” convert cytidines within a window of approximately five nucleotides (nt), and can efficiently correct a variety of point mutations relevant to human disease. In four transformed human and murine cell lines, second- and third-generation base editors that fuse uracil glycosylase inhibitor (UGI), and that use a Cas9 nickase targeting the non-edited strand, manipulate the cellular DNA repair response to favor desired base-editing outcomes, resulting in permanent correction of ~15-75% of total cellular DNA with minimal (typically ≤ 1%) indel formation. Base editing expands the scope and efficiency of genome editing of point mutations."